2’-Fluoro-RNA (2’-FRNA) contains a highly electronegative fluorine molecule at the 2’-ribose position resulting in a ring with C3’-endo conformation. Therefore, the oligonucleotide adopts stable A-form helix on hybridization to a target. This modification is particularly useful as antisense molecules, aptamers, siRNAs and miRNAs molecules. We offer 2’-Fluoro-RNA with phosphodiester or phosphorothioate linkages. Phosphorothioate bonds are phosphodiester analogues in which the non-bridging oxygen has been replaced by sulphur, increasing resistance against nuclease digestion.